Identification of Peanut Hybrids Using Microsatellite Markers and Horizontal Polyacrylamide Gel Electrophoresis
نویسندگان
چکیده
منابع مشابه
Hybrids in fishes and their identification using microsatellite DNA markers: A review
Among vertebrates, most of the fishes are unique for the lack of acrosome in their spermatozoa. The sperm enters the egg through micropyle of the egg. This has facilitated heterologous fertilization in many fishes and led to hybridization and polyploidization. Hybridization is performed both in the nature and domesticated fishes. Today, some of the hybrids have high growth rate than their paren...
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staining. However, these visualization methods require either expensive or hazardous radioactive chemicals and Microsatellite DNA markers are widely used in genetic research. are time-consuming. Electrophoresis with MetaPhor Their use, however, can be costly and throughput is sometimes limited. The objective of this paper is to introduce a simple, low-cost, highagarose gels (Cambrex Corporation...
متن کاملPolyacrylamide Gel Electrophoresis
Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...
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MATERIALS 5x TBE electrophoresis buffer Polyacrylamide gels are poured and run in 0.5x or 1x TBE at low voltage (1-8 V/cm) to prevent denaturation of small fragments of DNA by Joulic heating. Other electrophoresis buffers such as 1x TAE (please see Agarose Gel Electrophoresis) can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much...
متن کاملIdentification of L Forms by Polyacrylamide - Gel Electrophoresis THEODORE
Crude membranes were obtained from L forms by allowing the cells to lyse in distilled water. The crude membranes were washed several times in distilled water, lyophilized, and extracted with phenol-acetic acid-water. The membrane proteins were separated electrophoretically in polyacrylamide gels run at pH 4.5. Electrophoretic patterns and densitometric tracings of the gels showed distinct, repr...
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ژورنال
عنوان ژورنال: Peanut Science
سال: 2008
ISSN: 0095-3679
DOI: 10.3146/ps07-109.1